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Ritmos biológicos ... Empty Ritmos biológicos ...

el Mar Ago 04 2015, 12:31
Sabiamos que la oscuridad nocturna -en los ritmos circadianos humanos- estimula la producción de melatonina por la pineal induciendo al sueño y que la histamina elevaba otra vez el estado alerta por la mañana pero no sabiamos como, parece ser que es activando los canales del ion calcio, al menos en ratones. La histamina, candidata a la señora del día como la melatonina lo es de la noche:


Histamine resets the circadian clock in the suprachiasmatic nucleus through the H1R-CaV1.3-RyR pathway in the mouse

Abstract

Histamine, a neurotransmitter/neuromodulator implicated in the control of arousal state, exerts a potent phase-shifting effect on the circadian clock in the rodent suprachiasmatic nucleus (SCN). In this study, we investigated the mechanisms by which histamine resets the circadian clock in the mouse SCN. As a first step, Ca2+-imaging techniques were used to demonstrate that histamine increases intracellular Ca2+ concentration ([Ca2+]i) in acutely dissociated SCN neurons and that this increase is blocked by the H1 histamine receptor (H1R) antagonist pyrilamine, the removal of extracellular Ca2+ and the L-type Ca2+ channel blocker nimodipine. The histamine-induced Ca2+ transient is reduced, but not blocked, by application of the ryanodine receptor (RyR) blocker dantrolene. Immunohistochemical techniques indicated that CaV1.3 L-type Ca2+ channels are expressed mainly in the somata of SCN cells along with the H1R whereas CaV1.2 channels are located primarily in the processes. Finally, extracellular single-unit recordings demonstrated that the histamine-elicited phase delay of the circadian neural activity rhythm recorded from SCN slices is blocked by pyrilamine, nimodipine and the knock-out of CaV1.3 channel. Again, application of dantrolene reduced but did not block the histamine-induced phase delays. Collectively, these results indicate that, to reset the circadian clock, histamine increases [Ca2+]i in SCN neurons by activating CaV1.3 channels through H1R, and secondarily by causing Ca2+-induced Ca2+ release from RyR-mediated internal stores

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